RNA-LNP Formulation training

RNA–lipid nanoparticles (RNA-LNPs) are nanoscale carriers built from four main lipid components: ionizable lipids, cholesterol, phospholipids, and PEGylated lipids. Their design enables the encapsulation and delivery of nucleic acids such as mRNA or siRNA.

Recognized as the current benchmark in nucleic acid delivery, RNA-LNPs offer several advantages:

• Maximize RNA encapsulation
• Shield fragile RNA molecules from enzymatic breakdown
• Promote efficient cellular internalization and release from endosomes
• Allow fine-tuning of particle size, charge, and composition to balance safety, efficacy, and biodistribution

These attributes made RNA-LNPs the cornerstone technology behind the rapid and successful deployment of mRNA-based COVID-19 vaccines.

Lipid nanoparticles (LNPs) are versatile carriers capable of encapsulating a wide range of hydrophilic, charged, or delicate biomolecules and nucleic acids, such as:

• mRNA, saRNA, CircRNA – used in vaccines and protein replacement therapies
• CRISPR-Cas components– including RNP complexes or mRNA combined with gRNA
• siRNA, miRNA, ASO  – for gene silencing applications in oncology and rare diseases
• DNA
• Small hydrophilic drugs
• Peptides / proteins

As part of the training, you can access our LNP starter kits—developed with CordenPharma—that replicate established RNA-LNP formulations, allowing you to start formulating right away

During the training, we provide an introduction to strategies for optimizing RNA-LNP specificity and biodistribution. Two main approaches are discussed:

1. Active targeting – In more advanced designs, LNPs can be functionalized with targeting moieties (e.g., antibodies, peptides, or aptamers) to guide delivery toward specific tissues or cell types. Although this strategy requires additional design, characterization, and validation steps, it enables greater precision and tissue selectivity.
2. Passive targeting – By tuning intrinsic LNP parameters such as particle size, lipid composition, and lipid ratios, it is possible to influence how nanoparticles naturally distribute within the body. This approach is relatively straightforward and widely used, though it generally relies on extensive screening to identify the most effective formulation profiles.

Yes. We can provide full support for Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) on top of TAMARA training. This ensures your TAMARA system is installed correctly, operates according to specifications, and performs reliably under real experimental conditions. Our team can guide you through the qualification process so you can integrate the system into your workflows with confidence and meet regulatory requirements.

Getting started is simple. The typical workflow follows five steps:

1. Get in touch — Share the context of your request and your training objectives.
2. Consult with our RNA-LNP specialist — We’ll review your goals in detail and propose an action plan
3. Receive a quotation — Tailored to your project scope
4. Start the training — We execute the program according to your request.