NANOPARTICLES REVIEWS

mRNA LNP is a promising approach to a wide range of novel pharmaceuticals, vaccines, and therapeutic solutions. Its groundbreaking potential became evident to the public during the COVID-19 pandemic with the astonishingly quick development of the mRNA LNP Vaccine. This innovative RNA technology combining Messenger ribonucleic acid (mRNA) and lipid nanoparticle (LNP) holds the unique potential…

The astonishing rapidity of COVID-19 mRNA vaccine development surprised the global community. One of the primary drivers behind this extraordinarily quick development is the adoption of an advanced drug delivery system: the ionizable lipid nanoparticle. These lipid nanoparticles have been specifically engineered for RNA delivery, with their effectiveness attributed to the inclusion of ionizable lipids…

In the ever-evolving landscape of modern medicine, researchers are constantly seeking innovative strategies to enhance the efficiency and precision of lipid nanoparticle drug delivery. One of the most promising breakthroughs in this field is the use of lipid-based nanoparticles for drug delivery. These versatile lipidic carriers are transforming the way drugs are administered, offering increased…

With more than 5.5 billion people vaccinated worldwide during the Covid-19 pandemic, mRNA vaccines have come up as a highly effective strategy to fight quickly emerging diseases.  This unique capability to provide a quick, safe, and efficient therapy encouraged all the major players in the pharmaceutical industry to explore their use for a wide array…

In the ever-evolving landscape of cancer-therapies, the convergence of cutting-edge nanoparticles technology and health innovations has brought forth a promising paradigm shift. The use of lipid-based nanoparticle, such as albumin or liposomes, for cancer therapies has recently been brought to the spotlight for their innovative way of accessing cancerous tissues. Come along with us on…

How to optimize lipid nanoparticle formulation?  In the field of pharmaceutical advancements, the optimization of lipid nanoparticle formulation (LNP) stands as a critical pursuit for the next generation of RNA vaccines and therapeutics. The success of the BioNTech and Moderna Covid-19 vaccines can be attributed to two primary factors: the capacity to produce RNA in…

Introduction Amongst the numerous nanoparticle manufacturing methods available, microfluidics synthesis has appeared as the gold standard thanks to its unique ability to synthesize uniform nanoparticle populations, both at very small and very large scales. This solvent-based synthesis process goes beyond the mere microfluidic mixing of solutions but also involves several post-processing steps which are crucial…

Introduction The development of advanced drug delivery systems, such as lipid nanoparticles (LNP) has revolutionized modern medicine. These sub-micron carriers, composed of a combination of lipids, offer a versatile platform for transporting an array of therapeutic agents: mRNA, siRNA, small drugs… A critical factor in optimizing their performance is the width of these nanoparticles which…

In the fast-paced biotechnology landscape, LNP characterization, and more generation characterization of lipid-based nanoparticles, such as liposomes and lipid nanoparticles (LNP) as become cornerstone for the efficient development of new therapies. LNP and Liposomes have emerged as versatile and promising tools for the delivery of genetic material and small molecules to target various locations within…

Lipid-based nanoparticles, or LNP, are submicron particles specifically engineered to protect nucleic acid from degradation and optimal delivery to the cells. Beyond their well-documented role in mRNA-based vaccines, LNPs possess versatility in transporting various oligonucleotides. In this article, we will focus on the use of lipid nanoparticles for siRNA, also know as small interfering RNA…

In the realm of cutting-edge medical advancements, the use of lipid nanoparticles for mRNA (messenger RNA) delivery have emerged as a groundbreaking tool for a new generation of therapeutics, as illustrated by the nobel prize awarded to Katalin Karikó and Drew Weissman for their work in the field. This revolutionary technology holds the potential to…

Breast cancer is one of the main causes of death in women population. While all genders are affected by this cancer, only 1% of diagnosed patients are men. Cardiovascular diseases and cancer are the top mortality causes in women and breast cancer represents 24.5% of all cancer diagnosed, resulting in nearly 700 000 deaths in…

Over the last few decades, the significance of lipid-based nanoparticles (LBNs) development has greatly increased in the realm of biomedicine. Among these LBNs, solid lipid nanoparticles (SLNs) are part of the most promising ones, due to their unique properties. Their development started to overcome some limitations met with traditional nanocarriers like liposomes, such as physicochemical…

As several kinds of lipid-based nanoparticles (LBNPs) are currently available for delivering small molecules, oligonucleotides, and peptide/protein drugs, this review aims at providing a comparison of the 2 most widely used ones: liposomes vs lipid nanoparticles (LNPs).Although mistaken for LNPs, Liposomes are only close cousins of them, rather than ancestors, and present few differences in…

The microfluidic synthesis of lipid nanoparticles has recently been brought to the spotlight following the success of the mRNA-LNP based COVID-19 vaccine. As a matter of fact, a majority of the final drug formulation characteristics such as delivery efficiency, cell uptake, toxicity and side effects are linked to the composition and physicochemical characteristics of the…

The recent progress of microfluidics mixing technologies (micromixers) has been key to the recent success of the covid-19 LNP-mRNA-based vaccines.Micromixers permit the very fine control of the mixing speed of 2 liquids phases, which is one of the key factors to determine the final lipid nanoparticles physiochemical parameters, as introduced in our LNP formation mechanism…

Lipid nanoparticles formation mechanism remains relatively not well understood despite their wide usage, and more generally of organic nanoparticles, for the delivery of drugs or oligonucleotides to specific part of the body. Considering the importance of the lipid nanoparticles physiochemical parameters to their toxicity, encapsulation and delivery efficiency, having a finer understanding of the chemical…

The recent development of lipid-based nanoparticles (LBNPs) technologies as a carrier for drug delivery was key to the successful development of mRNA-based COVID-19 vaccine technologies and allowed unprecedented achievement at a time of urgent medical need. The high versatility, stability, and biocompatible nature of the LBNPs have permitted the delivery of a variety of bioactive…

Numerous methods are available for the synthesis of Lipid nanoparticles (LNP) and more generally for drug-loaded liposomes. The selected LNP formulation technique will depend on the sought characteristics of the synthesized drug (size, encapsulation efficiency, zeta potential), route of administration, development stage of the molecule… The LNP manufacturing methods can be categorized into 3 different…

Introduction to lipid nanoparticles Resulting of 50 years of research in the nanocarrier delivery field, lipid nanoparticles are close cousins to other lipid-based nanoparticles such as liposome and solid lipid nanoparticle (SLN). Unlike those which are specialized in the encapsulation of hydrophobic and/or hydrophilic small molecules, LNP have the unique capability of encapsulating nucleic acids…